Search academic texts

Information × Registration Number 0217U000854, 0116U004919 , R & D reports Title The development and trial exploitation of a portative analyzer based on amperometric enzyme biosensors for quality control of beverages in winery. Development of biosensor methods of arginine analysis in wine by using arginine-hydrolyzing recombinant enzymes. popup.stage_title Head Sibirny Andrey, Registration Date 10-03-2017 Organization Institute of Cell Biology NAS of Ukraine popup.description2 The L-arginine (Arg) selective enzymes - arginase and arginine deiminase (ADI) were isolated from the microbial cells - over producers of correspondent enzymes and characterized. The new enzymatic-chemical methods of Arg assay based on ADI, o-phthalaldehyde and 2,3-butanedionemonooxime (ADI-OPA and ADI-DMO methods, respectively) with spectrophotometric and fluorometric modes of detection were developed and tested . The proposed ADI-OPA method was tested on real samples of wines and juices in comparison with enzymatic (based on arginase I) and referent chemical methods. One of the developed method (ADI-OPA) was shown to be suitable for accurate and selective analysis of Arg level in drinks. The proposed methods correspond to the best foreign prototypes. According to the results of research 6 articles, one patent of Ukraine and 4 abstracts were published. Product Description popup.authors Іваш М.Ф. Гайда Г.З. Гончар М.В. Закальська О.М. Закальський А.Є. Стасюк Н.Є. Фаюра Л.Р. popup.nrat_date 2020-04-02 Close