Registration Number
0217U000854,
0116U004919
, R & D reports
Title
The development and trial exploitation of a portative analyzer based on amperometric enzyme biosensors for quality control of beverages in winery. Development of biosensor methods of arginine analysis in wine by using arginine-hydrolyzing recombinant enzymes.
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Head
Sibirny Andrey,
Registration Date
10-03-2017
Organization
Institute of Cell Biology NAS of Ukraine
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The L-arginine (Arg) selective enzymes - arginase and arginine deiminase (ADI) were isolated from the microbial cells - over producers of correspondent enzymes and characterized. The new enzymatic-chemical methods of Arg assay based on ADI, o-phthalaldehyde and 2,3-butanedionemonooxime (ADI-OPA and ADI-DMO methods, respectively) with spectrophotometric and fluorometric modes of detection were developed and tested . The proposed ADI-OPA method was tested on real samples of wines and juices in comparison with enzymatic (based on arginase I) and referent chemical methods. One of the developed method (ADI-OPA) was shown to be suitable for accurate and selective analysis of Arg level in drinks. The proposed methods correspond to the best foreign prototypes. According to the results of research 6 articles, one patent of Ukraine and 4 abstracts were published.
Product Description
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Іваш М.Ф.
Гайда Г.З.
Гончар М.В.
Закальська О.М.
Закальський А.Є.
Стасюк Н.Є.
Фаюра Л.Р.
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2020-04-02
