Registration Number
0217U000967,
0112U000614
, R & D reports
Title
Changes in expression of genes and activity of enzymes involved in carbohydrate and energy metabolism in malignant cells as mean of tumor growth suppression
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Head
Sibirny Andriy Andriyovych,
Registration Date
23-03-2017
Organization
Institute of cell biology, National Academy of Sciences of Ukraine
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The protocols for a highly selective and sensitive enzymatic determination of glucose using modified kit "DIAGLYUK-2" (using glucose oxidase and peroxidase and a highly sensitive chromogen) have been developed. Moreover, a new diagnostic kit "LACTATEST" for L-lactate analysis (using flavocytochrome b2 (L-lactate: cytochrome c-oxidoreductase) has been created at the Department of Analytical Biotechnology, Institute of Cell biology NAS of Ukraine. The developed methods were adapted to the analysis of the cultural liquid and cell-free extracts of tumors. The proposed methods were used to determine glucose and L-lactate in the cultural liquid (both substances) and cell-free extracts (L-lactate) of human myeloma, line RPMI 822. The detected production of L-lactic acid in the culture of myeloma confirms a Warburg phenomenon for this type of cancer cells. It was shown that bromopyruvate (inhibitor of glycolysis) in a concentration of 25 µM reduces the rate of glucose uptake. There is a tendency of decreased production of lactic acid in the culture of the human myeloma RPMI 822 as a consequence of the bromopyruvate treatment. In the frames of milestone for 2013 year, there were published 1article in international journal, 2 abstracts and submitted 2 papers.
Product Description
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Іваш М.Ф.
Гайда Г.З.
Гончар М.В.
Закальська О.М.
Закальський А.Є.
Куцяба В.І.
Лаврик М.І.
Прокопів Т.М.
Смуток О.В.
Стасюк Н.Є.
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2020-04-02
