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Information × Registration Number 0217U000967, 0112U000614 , R & D reports Title Changes in expression of genes and activity of enzymes involved in carbohydrate and energy metabolism in malignant cells as mean of tumor growth suppression popup.stage_title Head Sibirny Andriy Andriyovych, Registration Date 23-03-2017 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 The protocols for a highly selective and sensitive enzymatic determination of glucose using modified kit "DIAGLYUK-2" (using glucose oxidase and peroxidase and a highly sensitive chromogen) have been developed. Moreover, a new diagnostic kit "LACTATEST" for L-lactate analysis (using flavocytochrome b2 (L-lactate: cytochrome c-oxidoreductase) has been created at the Department of Analytical Biotechnology, Institute of Cell biology NAS of Ukraine. The developed methods were adapted to the analysis of the cultural liquid and cell-free extracts of tumors. The proposed methods were used to determine glucose and L-lactate in the cultural liquid (both substances) and cell-free extracts (L-lactate) of human myeloma, line RPMI 822. The detected production of L-lactic acid in the culture of myeloma confirms a Warburg phenomenon for this type of cancer cells. It was shown that bromopyruvate (inhibitor of glycolysis) in a concentration of 25 µM reduces the rate of glucose uptake. There is a tendency of decreased production of lactic acid in the culture of the human myeloma RPMI 822 as a consequence of the bromopyruvate treatment. In the frames of milestone for 2013 year, there were published 1article in international journal, 2 abstracts and submitted 2 papers. Product Description popup.authors Іваш М.Ф. Гайда Г.З. Гончар М.В. Закальська О.М. Закальський А.Є. Куцяба В.І. Лаврик М.І. Прокопів Т.М. Смуток О.В. Стасюк Н.Є. popup.nrat_date 2020-04-02 Close