Registration Number
0217U006292,
0116U000238
, R & D reports
Title
Set genus- and species-specific genetic markers the main pathogens of viral infections diseases carnivorous and fur animals (myxoma virus, caliсivirus, parvovirus, paramyxovirus, adenovirus, coronavirus) based on molecular genetic technology
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Head
Gerilovych Anton Pavlovych,
Registration Date
15-03-2017
Organization
Nationali Scientific Center "Institute of Experimental and Clinical Veterinary Medicine"
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Formed database sequencing of genomic material, the major genes and fragments thereof, new (since 2012 ..) isolates of pathogens parvovirus enteritis of dogs, cats feline panleukopenia, Aleutian Disease. A search in the database GenBank, EMBL, DDBJ sequences of genes parvovirus carnivores. It was found that the database contains information on about 200 partially or fully sequencing of gene sequences and the genomic DNA of the virus panleukopenia Cats (Feline panleukopenia virus, FPV), 600 - parvovirus dogs (Canine parvovirus, CPV), about 2700 sequences - virus Aleutian Disease (Aleutian mink disease parvovirus, AMDPV). Created database: 1) FPV - completely sequenced gene sequences and the VP2 VP1, encoding structural proteins, about 2,100 base pairs in length (bp) and 1600 bp according; Genomic DNA of about 4600 bp (Total 67 sequences) 2) CPV - completely sequenced and sequences of genes VP2 NS-1 encoding nonstructural proteins, about 1900, and 2000 bp according; Genomic DNA of about 4500 bp (Total 118 sequences) 3) AMDPV - completely sequenced gene sequences VP1, VP2, NS-1, the length of about 2200 bp, 1700 bp and 2,000 bp according; Genomic DNA of about 5000 bp (Total 183 sequences). All sequences were obtained in FASTA format (* .fasta) or GenBank (* .gb), which will use modern molecular biological software (including mode on-line) for the pair and plural, local and global alignment to determine conserved and variable gene fragments. Created primer system based on conserved protein genes for display and identification of pathogens parvovirus infection carnivore
Product Description
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Келеберда Микола Іванович
Кузнецов Євген Петрович
Рудова Наталія Генадіївна
Солодянкін Олексій Сергійович
Фісенко Світлана Анатоліївна
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2020-04-02
