Registration Number
0220U100564,
0115U004044
, R & D reports
Title
The metabolic and genetic aspects of viral infections in children and the modern methods of correction
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Head
Незгода Ірина Іванівна,
Registration Date
21-01-2020
Organization
Vinnytsia National Pirogov Memorial Medical University
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Objective: to estimate pathogenetic approaches to viral infections treatment based on the study of biochemical, metabolic, dysbiotic processes and genetic factors.
Objects of the study: pathogenetic mechanisms of rotavirus infection (RVI) development in children, clinical and laboratory patients’ characteristics with infectious mononucleosis (IM); chronic viral hepatitis B (CVH-B) in lymphoblastic leukemia (LBL) remission.
Methods: general clinical; instrumental; bacteriological; serological; molecular genetic; gas-liquid chromatography (GLC); immunological; analytical and statistical.
Firstly in children with RVI, VFA were identified by the GLC-method. Metabolic imbalance due to RVI is associated with depletion of the total VFA pool (0.048 ± 0.008 mg / ml) compared with healthy children (0.803 ± 0.34 mg / ml) at p <0.01, and significant deficiency of butyric fatty acid (0.017 ± 0.005 mg / ml, p <0.01). It was proved that children with T / T-13910 genotype of the LCT gene have more favorable course of the disease, and children with C / C-13910 and C / T-13910 genotypes of the LCT gene have more susceptibility to difficult course of the disease up to 1.2 times. The effectiveness of Saccharomyces boulardii and lactose-free diet in the treatment of RVI were substantiated.
Tonsils dysbiotic disorders due to IM were characterized by increasing role in the proportion of Streptococcus, Staphylococcus, Enterococcus, Pseudomonas, Enterobacter, Escherichia, Klebsiella. The high sensitivity of microflora to decamethoxin had been proved. One of the severity criteria of IM in children was a 5-fold increasing of lactoferrin level and twice increasing of α-interferon-I level.
Estimated that CVH-B runs over in the form of primary-chronic form with elevated levels of cytolytic enzymes (ALT - 78%, p <0,05). Liver fibrosis of F1-F3 level were diagnosed in 68.3% of children with CVH-B and background of LBL remission. The diagnostic value of osteopontin (OPN) was firstly identified.
Product Description
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Asaulenko Alla A.
Bobruk Svitlana V.
Levytska Lidiya I.
Nikulchenko Oleg V.
Naumenko Olha M.
Onofriichuk Olena S.
Singh Shailendra S.
Stanislavchuk Larysa M.
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2020-04-02
