Registration Number
0220U100921,
0119U101733
, R & D reports
Title
Development of a breast cancer cell screening system based on the 3D model of tumor spheroids enriched with cancer stem cells
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Head
Sydorenko Mykhailo V.,
Registration Date
29-01-2020
Organization
Department for special training National academy of science of Ukraine at Kiev University
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The object of the study is multicellular tumor spheroids of breast cancer cells of the MCF-7 line, enriched with cancer stem cells (CSCs). The purpose of the study is to assay a cell culture system of multicellular tumor spheroids enriched with cancer stem cells (eMCTS) to determine the effect of anticancer drugs. Research methods of the study are cultivation of adhesive cells and multicellular tumor spheroids (MCTS), immunohistochemical, morphometric, spectrophotometric, cell survival analysis (MTT test), counting of the cells in Goryaev's chamber. Increasing expression of CSCs markers in the eMCTS culture was shown, compare with the adhesive culture of MCF-7 cells, which were cultured under standard conditions. It has been determined that the eMCTS culture is less sensitive to the effects of anticancer drugs (cisplatin, methotrexate and doxorubicin), compared with MCTS and monolayer culture. It has been established that cells can acquire stem phenotype and lose it depending on the conditions in which they are found. The processes of peroxidation and neutralization of free radicals in the eMCTS are suppressed in comparison with MCTS, that non-enriched with CSCs. Intensification of the pentose phosphate pathway and inhibition of the glycolysis reactions in cells of eMCTS, compared with cells of monolayer cell culture and MCTS, are also shown. The use of eMCTS culture will allow us to investigate the effect of complex antitumor therapy on CSC and tumor cell populations.
Product Description
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Dzubenko Natalya V.
Kuznezova Galyna M.
Perepelytsina Olena M.
Rybalchenko Volodymyr K.
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2020-04-02
