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Registration Number

0221U101865, 0116U002453 , R & D reports

Title

Scientific substantiation of criteria for assessing and maintaining the biological activity of industrial microorganisms

Head

Danylenko Svitlana H, Доктор технічних наук

Registration Date

26-01-2021

Organization

Institute of Food Resources of the National Academy of Agrarian Sciences of Ukraine

Description

The aim of this work is to develop modern biotechnological approaches to the conditions of functioning and preservation of biomass of commercial crops intended for use in technologies of a wide range of national dairy and fermented meat products. the development is aimed at maintaining strains of various taxonomic groups in a viable state, preserving their useful properties for use in industry and storage of the collection by optimizing the conditions for lyophilization and cryopreservation To prevent the death of bacterial cells during freezing, the necessary protective media containing cryoprotectants, as a rule, carbohydrates and protein compounds. Sugars, penetrating into cells and creating a high osmotic pressure there, prevent the formation of ice crystals and cell destruction during the freezing process. Protein components are added to the medium to strengthen the connection of the cell membrane with the cytoplasm, which is important for thawing and rehydration of cells. It has been shown that bacteria stored frozen can retain their viability for a long time. The practice of developing protective media shows that in order to minimize cell death and product waste in terms of physical properties, the composition of a xeroprotector for each type of bacteria must include a qualitatively and quantitatively balanced set of components. 

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Anisimova Liudmyla V.
Harbar Nadiia I.
Danylenko Svitlana H.
Potemska Oksana I.
Ushakova Viktoriia V.
Khonkiv Myroslav O.