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Registration Number

0221U102172, 0120U101706 , R & D reports

Title

Intensification of butanol accumulation using different types of plant raw materials as substrate and domestic producing strains

Head

Shulga Sergey M., Кандидат фізико-математичних наук

Registration Date

28-01-2021

Organization

Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine

Description

 The subject of the study were the butanol-producing strain Clostridium sp. ІМВ В-7570 (IFBG C6H 5M) from the “Collection of strains of microorganisms and plant lines for agricultural and industrial biotechnology” SE “Institute of Food Biotechnology and Genomics of the NAS of Ukraine” and biomass of 4 varieties of sugar sorgum (MM Grishko National Botanic Garden of the National Academy of Sciences of Ukraine). The aim of this work is to conduct preliminary preparation of raw materials using different types of hydrolysis and determine the optimal for the accumulation of butal. Research methods: psychochemical, biological. Pretreatment of raw materials using different types of hydrolysis was carried out and the optimal for the accumulation of butanol was determined. It is shown that the optimization of the conditions of raw materials pretreatment increased the accumulation of butanol by 25-50%. It was determined that the combination of acid and enzymatic hydrolysis turned out to be the optimal pretreatment for the sorghum biomass of sugar varieties Energodar and Amber 1 India. It was determined that the combination of alkaline and enzymatic hydrolysis turned out to be the optimal pretreatment for the sorghum biomass of sugar varieties Alta seads and St-20.

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popup.authors

Andriiash Ganna S
Beiko Natalia Ye.
Tigunova Helen O.
Shulga Sergey M.