Registration Number
0221U107015,
0120U000107
, R & D reports
Title
Development of arginine-selective sensors for the analysis of food and dietary supplements used in preventive and rehabilitation medicine
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Head
Saiapina Olha Ya,
Registration Date
26-12-2021
Organization
Institute of Molecular Biology and Genetics of NAS of Ukraine
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The main aim of the project was to develop highly sensitive and selective electrochemical bio- and chemosensors for determination of arginine and to optimize the composition of their (bio)selective elements and parameters of the working solutions for the further application in the analysis of food and dietary supplements used in the diet of therapeutic, prevention and rehabilitation purposes. At the second stage of research, a laboratory prototype of a monoenzyme conductometric biosensor based on the recombinant arginine deiminase from Mycoplasma hominis was developed. The composition and conditions of immobilization of bioselective elements of the biosensor were selected; the influence of parameters of the working buffer solution on the sensitivity of the biosensor to arginine was studied in detail. The analytical characteristics and selectivity of the biosensor when working with model solutions of arginine were studied in the optimized buffer solution.
Performance of all bio- and chemosensors developed during two stages of the project was analyzed, and their most successful types were selected for further testing in the analysis of real samples. The conductometric biosensor based on arginase, urease and clinoptilolite and the biosensor based on arginine deiminase were used to determine the concentration of arginine in 15 samples of food and dietary supplements. Results of the biosensor analysis showed high correlation with the data of reference methods, except for some samples. Thus, the highly sensitive, reliable and easy to manufacture conductometric biosensors for determination of arginine in food and dietary supplements were developed and can be considered as the promising analytical tools for use in a comprehensive procedure of the quality control of products, which contain arginine. The procedure of analysis using the developed biosensors is characterized by high speed, low cost and suitability for use in both centralized laboratories and in the field.
Product Description
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Kucherenko Daria Yu.
Nesterenko Yevheniya О.
Saiapina Olha Ya.
Soldatkin Oleksandr O.
Yarynka Dariia V.
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2022-03-09
