Registration Number
0221U107195,
0121U110360
, R & D reports
Title
Molecular mechanisms of vitamin D-auto/paracrine system involvement in the regulation of cellular effects of SARS-CoV-2 S protein.
popup.stage_title
Head
Lisakovska Olha O., Кандидат біологічних наук
Registration Date
29-12-2021
Organization
Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine
popup.description2
Using the genetic constructs for recombinant expression pET28a-SARS-CoV-2-Spike-RBD and pET28b-mNG-RBD in the prokaryotic expression system, a recombinant receptor-binding domain (RBD) of the S-protein of SARS-CoV-2 and labeled with fluorescent protein mNeonGreen were obtained, and were purified by immobilized-metal affinity chromatography. It was shown that the metabolic activity of MA-104 cells (kidney epithelium of green african monkey), was decreased after 1.25(OH)2D3 action in the concentration range of 0.78-50 nmol/L, while the metabolic activity of pig testicular epithelial cells (ST-cells) was inhibited only at the lowest concentration of 1.25(OH)2D3 - 0.78 nmol/L. It was demonstrated that 1.25(OH)2D3 in the range of 25-100 nmol/L increased the percentage of binding of RBD-FITC and RBD-mNeonGreen to ACE2 on the surface of MA-104 cells and did not affect the binding of RBD-FITC-ACE2 complex on ST-cells. Finally, molecular mechanisms that can mediate the protective action of vitamin D3 during coronavirus disease may include: decreased production of reactive oxygen species, lowered expression of the enzyme glyoxylase 1, which is responsible for the utilization of methylglyoxal, inhibition of p38 and NF-κB signaling pathways (on the mRNA and protein levels), as well as an increase in the content of NF-κB inhibitor – IκB.
Product Description
popup.authors
Krynina Olha I.
Mazanova Anna Oleksandrivna
Tykhonenko Tetiana M.
popup.nrat_date
2022-03-09
