Registration Number
0222U000013,
0121U113765
, R & D reports
Title
Development of the method for producing active pharmaceutical ingredients with anti-inflammatory and antioxidant activity based on wormwood root biotechnology
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Head
Matvieieva Nadiia A., Доктор біологічних наук
Registration Date
01-01-2022
Organization
Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine
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To develop a method for obtaining active pharming ingredients from "hairy" wormwood roots, work was carried out to study the physiological and biochemical characteristics of the root lines available in the collection. Peculiarities of growth of "hairy" wormwood roots of two species have been established, variability in growth rate has been revealed. Based on the analysis of the total content of flavonoids and the level of antioxidant activity, samples of "hairy" roots - potential producers of bioactive compounds - were selected. It was determined that, despite the intensification of the growth of "hairy" roots when cultivated on a nutrient medium with reduced nitrogen content, it is advisable to use the main medium Murashige and Skoog due to reduced specific flavonoids with reduced nitrogen concentration.
The method of obtaining extracts was optimized, the parameters of HPLC analysis of qualitative and quantitative content of flavonoids in extracts were determined, the presence of 12 flavonoids was established, in particular, those known for high levels of antioxidant and anti-inflammatory activity, namely quercetin, apigenin, luteolin and polyphenolic acids. gallic acid). The analysis of anti-inflammatory activity on the ability of extracts to inhibit 15-LOX was performed and the dose-dependence of such activity was revealed. The PCR technique with different pairs of primers was optimized.
Product Description
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Bessarabov Volodymyr I.
Bohdanovych Taisa A.
Duplij Volodymyr P.
Kobylinska Nataliia H.
Morgun Bogdan V.
Ratushniak Yakiv I.
Shakhovsky Anatolij M.
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2022-03-09
