Registration Number
0222U001080,
0121U107434
, R & D reports
Title
Study of the features of the influence of binary radiation technologies on human malignant cells in vitro
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Head
Lavrenchuk Halyna Yo., д.б.н.
Talko Victoriia V., Доктор медичних наук
Registration Date
23-01-2022
Organization
State Institution "National Research Centre For Radiation Medicine of National Academy of Medical Sciences of Ukraine"
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Object of research: biological effects in cultures of normal and malignant human cells under the action of different concentrations of photon-capturing agent and photosensitizer. Purpose: to investigate structural and morpho-functional changes in test systems of malignant (line A-549) and normal (fibroblasts 6 passages) human cells during incubation with gadolinium-containing photon-capture agent "Dotavist" and photosensitizer "Photolon". Research methods: method of transplanted cell culture of normal human fibroblasts and malignant human cells, cytological, biophysical, statistical.The study of morphofunctional characteristics of test systems during incubation with gadolinium-containing photon-capture agent "Dotavist" in a wide range of concentrations (5, 10, 25, 50, 100 and 200 μl / ml) found that at low concentrations of 5 ÷ 25 μl / ml proliferative and mitotic activity did not differ from control. At concentrations of 100 and 200 μl / ml, destruction of the cell monolayer, structural changes in cell culture and the appearance of atypical cells in culture were observed. Studies with the photosensitizer "Photolon" showed opposite effects of morphofunctional characteristics of cell cultures: cytotoxic effects on malignant cells A-549 were manifested at the lowest concentrations of 5 and 10 μl / ml.Changes in the culture of normal fibroblasts under the action of FS in the concentration range of 5 ÷ 200 μl / ml indicate the genotoxic properties of the photosensitizer on normal human fibroblasts.
Product Description
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Atamaniuk Nataliya P
Dmytriyeva Inna R
Malychevska Evgueniya M
Potchapinskiy Oleksiy D
Chernyshov Andriy V
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2022-03-09
