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Registration Number

0222U001081, 0120U100747 , R & D reports

Title

Study of molecular-genetic features of pathogenesis of Ph-negative myeloproliferative neoplasia in persons who have been exposed to ionizing radiation due to the Chernobyl accident

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Head

Neumerzhytska Liubov V., Кандидат біологічних наук

Registration Date

23-01-2022

Organization

State Institution "National Research Centre For Radiation Medicine of National Academy of Medical Sciences of Ukraine"

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 Object of research - genomic DNA and medical history maps with a diagnosis of Ph-negative myeloproliferative neoplasia (MPN) The aim of the study was to investigate the frequency of JAK2 mutations, to identify variants of nucleotide sequences in epigenetic regulation genes in patients with Ph-negative MPN with a history of radiation exposure and to determine their prognostic significance in vascular pathology. Research methods - molecular genetic (PCR), analytical, statistical. It was found that patients with a history of radiation are more likely to have a negative status for the JAK2 mutation than those who became ill spontaneously with a detected frequency of (24.41 ± 1.69)% and (37.82 ± 7.66)%. There are more cases of mutation in men than in women in both groups. Thrombotic complications predominate in the group of patients with a history of radiation. No correlation was found between the presence of mutations and the frequency of thrombotic events in patients in each group. Thrombocytosis in men is directly correlated with age, in women there is a bimodal distribution by age. In groups of patients with radiation history and triple-negative mutational status found more additional nonspecific mutations in the genes of epigenetic regulation DTA (DNMT3A, TET2, ASXL1) and increased thrombotic complications: with the presence of mutations - (55.5 ± 16.5)%, without mutations - (15.4 ± 10.1)%. indicating that the V617F mutation of the JAK2 gene is not the only key molecular genetic marker of Ph (-) MPN in the formation of thrombosis.

Product Description

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VERBYLENKO ROKSANA MYKOLAYIVNA
KLIMUK BOHDANA TARASIVNA
KLYMENKO SERHII VIKTOROVYCH
NEUMERZHYTSKA LIUBOV V

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2022-03-09