Registration Number
0222U002091,
0117U000786
, R & D reports
Title
Enhancement the efficiency of conventional and experimental drugs in vitro and in vivo through their immobilization on nanoscale carriers and such carriers combined with antioxidants
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Head
Stoika Rostyslav S., Доктор біологічних наук
Registration Date
09-02-2022
Organization
Institute of Cell Biology of the National Academy of Sciences of Ukraine
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We have investigated the biochemical parameters of hepato-, cardio- and nephocytotoxicity of antitumor drugs complexed with poly-2-oxazoline carrier on laboratory mice, as well as on mice with inoculated tumors. Changes in the leukogram and erythrocyte content of experimental mice were also studied.
The ability of new 4-thiazolidinone derivatives, and thiazole derivatives in free form and immobilized on synthetic polymeric carriers, to induce apoptosis and disrupt DNA structure in mammalian tumor cells has been studied.
We have obtained a preparative amounts of shrimp chitosan and chitosan-melanin complex of bees, as well as fragments of chitosan of medium (150 kDa) and low (50 kDa) molecular weight for their application as carriers of drugs and other ligands. For the first time we have established the influence of counter-anions of inorganic and organic nature in salt forms of chitosans on their biological activity and physical and chemical characteristics.
The results of cytological studies of the toxicity of chitosan and its derivatives on different cell types allowed us to determine the most promising derivatives with antibacterial, antifungal action, as well as the level of their toxicity towards different types of animal cells. We hav analyzed the changes in the level of antitumor and antimicrobial effect due to increased water solubility of synthetic antitumor substances in the composition of the created complexes and/or prolongation of their action.
The molecular mechanisms of cytotoxic action of Ser-Pro-Cys peptide nanoconjugates towards human malignant tumor cells in vitro have been studied.
Deletion analysis and site-specific mutagenesis of the 3’-untranslated region (3’-UTR) cDNA of the key transcription factor Smad3 of the TGF-β signaling pathway have been performed. This revealed the molecular mechanisms of hyperactivation of this signaling pathway after its hypersensitivity by hyperthermia and the action of certain antitumor drugs.
Product Description
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Ivasechko Iryna Ihorivna
Antoniuk Volodymyr Oleksandrovych
Кit Yuriy Yaroslavovych
Kashchak Nataliya I.
Klyuchivska Olga Yu.
Kozak Yuliia S.
Korchynskiy Oleksandr H.
Lootsik Maxim D.
Manko Nazar Olegovych
Panchuk Rostyslav R.
Senkiv Yuliya V.
Skorokhyd Nadiya R.
Starykovych Marina O.
Stoika Rostyslav S
Finiuk Nataliya S.
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2022-03-09
