Registration Number
0223U000782,
0120U101644
, R & D reports
Title
Pharmacological modulation of glutathione-dependent regulation and implementation mechanisms of endogenous neuroprotection and neuroplasticity - a new perspective on neuroprotection in ischemic strokes
popup.stage_title
Head
Byelenichev Ihor F., Доктор біологічних наук
Registration Date
20-01-2023
Organization
Zaporizhzhya State Medical University
popup.description2
The object of study is HSP70/GSH-dependent mechanisms of endogenous neuroprotection in cerebral ischemia. The purpose of
the study – an experimental substantiation of administration of modulators of the glutathione system (glutathione,
glutaredoxine and selenium derivatives: Se-glutathione, Se-methionine, Se-cysteine), as well as receptor antagonist of
interleukin-1β (RAIL, IL-1Ra) and heat shock factor 1 (HSF-1) in the treatment of stroke based on the basis of experimental data
revealing the role of glutathione systems in HSP70-mediated mechanisms of regulation of endogenous neuroprotection.
Research methods – molecular, morphometric, biochemical, histochemical, polymerase chain reaction and immunoblotting. The
results of the Scientific research work and its novelty. For the first time a scientific direction was formed to solve the
fundamental problem of pharmacology – the development and production of new drugs for the treatment of cerebrovascular
diseases – the complex methodology for creating intranasal systemic drugs with neuroprotective activity in the dosage form of
liquid gels was developed. This is the new and advanced mechanism of RAIL action based on the concept of target
neuroprotection. HSP70 blocks the trigger receptors of apoptosis (Fas / Apo-1), inhibits apoptosis in mitochondria at the stage
between release of cytochrome C and cleavage of procaspase-9, preventing binding of cytochrome C with the proapoptotic
protein Apaf-1 in mitochondria. HSP70 is able to repair oxidatively damaged antioxidant enzymes and transport them from the
cytoplasm to other cellular organelles. Increase in expression and/or levels of intracellular HSP70 produces inhibition of
transmission of proliferative, apoptotic, inflammatory signals. HSP70 has direct mitoprotective action, and supports the activity
of compensatory malate-aspartate shuttle mechanism under conditions of cerebral ischemia, as well as prolongs the action of
HIF-1a
Product Description
popup.authors
Aliieva Olena Н.
Bak Pavlo H.
Berest Halyna Hryhorivna
Burlaka Bohdan S.
Bukhtiiarova Nina V
Hanzhuk Yana B.
Zidrashko Halyna A.
Zavhorodnia Maryna
Makieieva Liudmyla V.
Nosach Svitlana H.
Popazova Olena O.
Pruhlo Yevhen Serhiiovych
Ryzhenko Viktor P.
Samura Iryna
Skorina Dmytro Yuriyovych
Tykhonovska Maryna A.
Tykhonovskyi Oleksandr V.
popup.nrat_date
2023-01-20
