Search academic texts

Information × Registration Number 0223U000782, 0120U101644 , R & D reports Title Pharmacological modulation of glutathione-dependent regulation and implementation mechanisms of endogenous neuroprotection and neuroplasticity - a new perspective on neuroprotection in ischemic strokes popup.stage_title Head Byelenichev Ihor F., Доктор біологічних наук Registration Date 20-01-2023 Organization Zaporizhzhya State Medical University popup.description2  The object of study is HSP70/GSH-dependent mechanisms of endogenous neuroprotection in cerebral ischemia. The purpose of the study – an experimental substantiation of administration of modulators of the glutathione system (glutathione, glutaredoxine and selenium derivatives: Se-glutathione, Se-methionine, Se-cysteine), as well as receptor antagonist of interleukin-1β (RAIL, IL-1Ra) and heat shock factor 1 (HSF-1) in the treatment of stroke based on the basis of experimental data revealing the role of glutathione systems in HSP70-mediated mechanisms of regulation of endogenous neuroprotection. Research methods – molecular, morphometric, biochemical, histochemical, polymerase chain reaction and immunoblotting. The results of the Scientific research work and its novelty. For the first time a scientific direction was formed to solve the fundamental problem of pharmacology – the development and production of new drugs for the treatment of cerebrovascular diseases – the complex methodology for creating intranasal systemic drugs with neuroprotective activity in the dosage form of liquid gels was developed. This is the new and advanced mechanism of RAIL action based on the concept of target neuroprotection. HSP70 blocks the trigger receptors of apoptosis (Fas / Apo-1), inhibits apoptosis in mitochondria at the stage between release of cytochrome C and cleavage of procaspase-9, preventing binding of cytochrome C with the proapoptotic protein Apaf-1 in mitochondria. HSP70 is able to repair oxidatively damaged antioxidant enzymes and transport them from the cytoplasm to other cellular organelles. Increase in expression and/or levels of intracellular HSP70 produces inhibition of transmission of proliferative, apoptotic, inflammatory signals. HSP70 has direct mitoprotective action, and supports the activity of compensatory malate-aspartate shuttle mechanism under conditions of cerebral ischemia, as well as prolongs the action of HIF-1a Product Description popup.authors Aliieva Olena Н. Bak Pavlo H. Berest Halyna Hryhorivna Burlaka Bohdan S. Bukhtiiarova Nina V Hanzhuk Yana B. Zidrashko Halyna A. Zavhorodnia Maryna Makieieva Liudmyla V. Nosach Svitlana H. Popazova Olena O. Pruhlo Yevhen Serhiiovych Ryzhenko Viktor P. Samura Iryna Skorina Dmytro Yuriyovych Tykhonovska Maryna A. Tykhonovskyi Oleksandr V. popup.nrat_date 2023-01-20 Close