Registration Number
0223U001323,
0119U103919
, R & D reports
Title
To investigate the efficiency of using tissue engeneering technology at the spinal cord damage in the experiment
popup.stage_title
Head
Tsymbaliuk Vitalii Ivanovych, Доктор медичних наук
Registration Date
27-01-2023
Organization
The State Institution Romodanov Neurosurgery Institute National Academy of Medical Sciences of Ukraine
popup.description2
Реферат (англійською мовою):
FIBRIN MATRIX, SPINAL CORD INJURY, REGENERATION, RESTORATION
TREATMENT, NEUTROTRANSPLANTATION.
The object of research is spinal cord injury.
The purpose of the study is to find out the effect of two-component neurotransplantation agentsbased on fibrin matrix on the course of spinal cord injury.
Left side cross-section of half of the spinal cord is modeled lower thoracic‒upper lumbar level and immediate implantation in the focus was carried outinjuries of human fibrin matrix associated with: 1) neonatal cells rat brain, 2) with mesenchymal progenitors of human Wharton cells, 3) with labeled mesenchymal progenitors of mouse subcutaneous adipose tissue, 4) with labeled neural progenitors of the fetal hippocampus of the mouse or 5) with brain neurotrophic factor. The level of motor function and spasticity is paretic the extremities were evaluated, respectively, according to the BBB scale and the Ashworth scale in their own technicians modifications. The morphological picture in the area of the injury was examined ~7 months later using impregnation of longitudinal sections of the spinal cord with silver nitrate and/or immunohistochemically.
It was found that the immediate implantation of the fibrin matrix in the focus of injury, associated with neonatal brain or mesenchymal cells by the progenitors of Wharton's jelly, as well as the implantation of a fibrin matrix enriched growth factor BDNF, - cause an equal and reliable positive effect on restoration of the motor function of the animal. Fibrin matrix implantation along with mesenchymal progenitors of adipose tissue, as well as fibrin matrix without no added cells, is accompanied by a statistically insignificant positive effect. Use as a cellular co-component of a neural transplant progenitors, significantly worsens the restorative effect, which may be caused by immunogenic factors mechanisms of leveling the effect of transplanted cells on the perifocal zone of the injury
Product Description
popup.authors
Yehorova Diana Mykhailivna
Vaslovych Viktoriya Viktorivna
VELICHKO Olga Mykolayivna
VERBOVSKA Svitlana Anatolyivna
DUNAYEVSKA Lyudmila Oleksandrivna
LYUBYCH Larisa Dmitrivna
MEDVEDYEV Volodymyr Viktorovych
Nakhaba Oleksandr Oleksandrovych
OLEKSENKO Natalia Pavlivna
PICHKUR Leonid Dmytrovych
STAYNO Larisa Petrivna
Tsymbaliuk Vitalii I.
SHEVCHUK Orysia Volodymyrivna
popup.nrat_date
2023-01-27
