Registration Number
0223U002012,
0122U000856
, R & D reports
Title
Cryopreservation of spheroids from stem / progenitor cells of different histogenetic origin based on physical and mathematical modeling.
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Head
Petrenko Oleksandr Yu., Доктор біологічних наук
Registration Date
07-02-2023
Organization
Institute of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine
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The object of the study is the osmotic characteristics of homogeneous and heterogeneous spheroids (SF) obtained from cells of different histogenetic origin (mesodermal – mesenchymal stem cells (MSCs) of an adult and the L929 line of mouse fibroblast-like cells; ectodermal – neural cells (NC) of the brain of newborn rats and derivatives of the neural crest from the spinal ganglia of newborn piglets). The aim of the work is to establish the main parameters of spheroid formation and morphofunctional profile for heterogeneous spheroids from stem/progenitor cells and to determine their osmotic characteristics in dimethylsulfoxide (DMSO) cryoprotectant solutions. Theoretically calculated filtration coefficients Lp and permeability coefficients kp for water and DMSO for a number of bioobjects (cells, SF, cells depending on localization in SF). It was found that for cells of the L929 line, the coefficients Lp and kp are the largest for cells in suspension and decrease for cells in the composition of a spheroid with an increase in the depth of their location. Optimal modes of cryopreservation of SF from L929 cells were calculated, depending on the terms of cultivation. The necessary conditions for the formation of spheroids from MSCs have been determined and the following parameters have been established: surface-to-volume ratio, osmotically inactive volume, Lp and kp coefficients for water molecules and DMSO cryoprotectant, values of the activation energy of their penetration. According to the calculations of physical and mathematical modeling, the range of optimal cooling rates of spheroids obtained from MSC is determined to be 0.75 ÷ 1 deg/min, the recommended concentration of DMSO is 7.5% (1 M).
Product Description
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Alabedalkharim Natalia M.
Bozhok Galyna A.
Vsevolodska Svitlana O.
Gordiyenko Olga I.
Kovalenko Igor F.
Kovalenko Svitlana Ye.
Legach Yevgen I.
Mazur Svitlana P.
Moiseyeva Natalia M.
Ochenashko Olga V.
Revenko Olena B.
Rogulska Olena Yu.
Sidorenko Olga S.
Sukach Oleksandr M.
Trufanova Natalia A.
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2023-02-07
