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Information × Registration Number 0223U005259, (0119U100821) , R & D reports Title Endogenous factors for the development of myeloprpliferative tumors and prostate cancers popup.stage_title Проаналізувати ефективність використання інгібіторів/активаторів клітинних процесів в запропонованих модельних системах. Розробка практичних рекомендацій. Заключний звіт. Head Telegeev Gennadiy D., Доктор біологічних наук Registration Date 19-12-2023 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description1 Study of factors leading to the onset of tumor progression in patients with myeloproliferative neoplasm (MPN) and prostate cancer popup.description2  Research object: BCR-ABL chimeric oncogene, BCR gene, BCR proteins, p190 BCR-ABL, p210 BCR-ABL, alkaline phosphatase, Vit E, C/EBP alpha, cortactin, USP1, prostate cancer. Methods: Standard molecular biological protocols, which included: isolation of RNA and DNA, oligonucleotide primers design, reverse transcriptase polymerase chain reaction (RT-PCR), PCR, real-time PCR, creation of recombinant DNA, Southern hybridization, DNA sequencing, fluorescence confocal microscopy of live and fixed mammalian cells, ultra-high-resolution fluorescence microscopy STED, etc. Work results. New interactions of BCR-ABL oncoprotein with USP1, GLG1 and ZFP217 proteins in Ph-positive cells of human chronic myeloid leukemia were established and characterized. It was shown that inhibition of USP1 deubiquitinase activity affects the level of BCR-ABL oncoprotein in K562 cells and a hypothetical model of ubiquitin-proteosomal degradation of BCR-ABL oncoprotein in CML was proposed. With the help of confocal and subdiffraction fluorescence microscopy STED, it was found that the BCR part of the BCR-ABL protein due to the PH domain co-localizes with cortactin and γ-tubulin in the centrosomal region at the sites of actin branching. The level of expression of epithelial-mesenchymal transition genes and antioxidant protection genes in prostate tumor cultures with different radioresistance was evaluated. It was shown that vitamin E suppresses the expression of the transcription factor Snail1, the main EMT inducer, the pluripotent transcription factor Okt4 and activates the expression of the transcription factor CEBP alpha. Product Description popup.authors Olha V. Zimina Dybkov Mykhaylo Vasylovych Tetшana Y. Lysets'ka Svitlana V. Antonenko Ihor V. Kravchuk Liudmyla P. Shvachko Lev О. Polishchuk Hurianov Dmytro S. popup.nrat_date 2023-12-19 Close