Registration Number
0223U005259,
(0119U100821)
, R & D reports
Title
Endogenous factors for the development of myeloprpliferative tumors and prostate cancers
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Проаналізувати ефективність використання інгібіторів/активаторів клітинних процесів в запропонованих модельних системах. Розробка практичних рекомендацій. Заключний звіт.
Head
Telegeev Gennadiy D., Доктор біологічних наук
Registration Date
19-12-2023
Organization
Institute of Molecular Biology and Genetics of NAS of Ukraine
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Study of factors leading to the onset of tumor progression in patients with myeloproliferative neoplasm (MPN) and prostate cancer
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Research object: BCR-ABL chimeric oncogene, BCR gene, BCR proteins, p190 BCR-ABL, p210 BCR-ABL, alkaline phosphatase, Vit E, C/EBP alpha, cortactin, USP1, prostate cancer.
Methods: Standard molecular biological protocols, which included: isolation of RNA and DNA, oligonucleotide primers design, reverse transcriptase polymerase chain reaction (RT-PCR), PCR, real-time PCR, creation of recombinant DNA, Southern hybridization, DNA sequencing, fluorescence confocal microscopy of live and fixed mammalian cells, ultra-high-resolution fluorescence microscopy STED, etc.
Work results. New interactions of BCR-ABL oncoprotein with USP1, GLG1 and ZFP217 proteins in Ph-positive cells of human chronic myeloid leukemia were established and characterized. It was shown that inhibition of USP1 deubiquitinase activity affects the level of BCR-ABL oncoprotein in K562 cells and a hypothetical model of ubiquitin-proteosomal degradation of BCR-ABL oncoprotein in CML was proposed. With the help of confocal and subdiffraction fluorescence microscopy STED, it was found that the BCR part of the BCR-ABL protein due to the PH domain co-localizes with cortactin and γ-tubulin in the centrosomal region at the sites of actin branching. The level of expression of epithelial-mesenchymal transition genes and antioxidant protection genes in prostate tumor cultures with different radioresistance was evaluated. It was shown that vitamin E suppresses the expression of the transcription factor Snail1, the main EMT inducer, the pluripotent transcription factor Okt4 and activates the expression of the transcription factor CEBP alpha.
Product Description
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Olha V. Zimina
Dybkov Mykhaylo Vasylovych
Tetшana Y. Lysets'ka
Svitlana V. Antonenko
Ihor V. Kravchuk
Liudmyla P. Shvachko
Lev О. Polishchuk
Hurianov Dmytro S.
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2023-12-19
