Registration Number
0223U005389,
0122U002240
, R & D reports
Title
The role of immunomodulatory in the antitumor action of traditional and experimental medicines at their delivery by polymeric nanocarriers to various target cells in vitro and in vivo
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Head
Stoika Rostyslav S., д.б.н.
Registration Date
22-12-2023
Organization
Institute of Cell Biology of the National Academy of Sciences of Ukraine
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As part of the 1st stage of the work, the in vivo toxic effect on laboratory mice of synthesized derivatives of pyrrolidinedione-thiazolidinone (compound Les-6485) and thiopyrano[2,3-d]thiazole (compound Les-6400) was evaluated. Under the influence of these compounds, mice of the C57BL/6 line did not lose weight and all animals survived, while the administration of doxorubicin to animals caused 50% death of animals, a decrease in their weight and significantly reduced the number of leukocytes, platelets, erythrocytes and hemoglobin in the peripheral blood of mice.
As part of the 2nd stage of the work, the low thiol reactivity of the pyrrolidinedione-thiazolidinone and thiopyrano[2,3-d]thiazole derivatives was indicated.
Within the framework of the 3rd stage of the work, a comparison of the ability of doxorubicin, oxaliplatin and experimental (4 thiosemicarbazone derivatives – triapine (3-AP), COTI-2, DpC, KP-2550) agents to induce immunogenic cell death in the B16 mouse melanoma model was carried out and also to prevent the development of this tumor in mice of the C57BL/6 line upon the introduction of a cellular vaccine from B16F10/wt melanoma cells treated with the indicated drugs. The life expectancy of the immunized animals, the size of their tumor, as well as the indicators of their hematological profile (leukocytes, erythrocytes, platelets, lymphocytes, neutrophils, monocytes, hemoglobin content) on the 30th and 120th day after re-inoculation of the tumor were determined.
As part of the 4th stage of the work, a method of obtaining a chitosan gel containing the antibiotic ampicillin, attached to chitosan by an ionic bond, was developed. Convenient methods for obtaining chitosan-based hydrogels stabilized by natural polyanionic polymers (hyaluronic acid and chondroitin-6-sulfate) have been developed. All components of the created hydrogels are non-toxic for cells of epithelial and connective tissues of mammals.
Product Description
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Ivasechko Iryna Igorivna
Antoniuk Volodymyr Oleksandrovych
Kashchak Nataliya Ivanivna
Klishch Mykola Vasylovych
Klyuchivska Olga Yuriivna
Kozak Yuliia S.
Lootsik Maxim Dmytrovych
Manko Nazar Olegovych
Panchuk Rostyslav R.
Senkiv Yuliya V.
Skorokhyd Nadiya Romanivna
Starykovych Marina O.
Stoika Rostyslav
Finiuk Nataliya
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2023-12-22
