Registration Number
0224U000495,
(0119U100441)
, R & D reports
Title
Study of mechanisms of cryoprotection of mammalian erythrocytes in the model of post-hypertonic shock and after freeze-thawing
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Вивчення механізмів кріопошкодження еритроцитів ссавців на моделі постгіпертонічного шоку і після розморожування
Head
Shpakova Nataliia M., Доктор біологічних наук
Registration Date
08-01-2024
Organization
Institute of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine
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The purpose is to study the features and regularities of the response of mammalian erythrocytes to post-hypertonic shock at the freeze-thawing stage and to determine the conditions, ensuring an increased cell resistance
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The object of research is mammalian erythrocytes. The research aim is to study the peculiarities and regularities of the reaction of mammalian erythrocytes to the action of posthypertonic shock at the thawing stage and to determine the conditions, which provide to enhance cell resistance. Research methods are spectrophotometry, pH-metry, osmometry, microscopy, ionometry, flow cytometry, methods of statistical processing of the results. It has been shown that sensitivity of mammalian erythrocytes to posthypertonic shock (PHS) is differing significantly. The dehydration stage is more important in development of PHS of studied mammalian erythrocytes than the rehydration one. The PHS level of mammalian erythrocytes is determined by the temperature of the medium, moreover at 0℃ the indicators are lower than at 37℃. Amphiphilic compounds belonging to different classes of surface-active substances and having different physico-chemical properties show high antihemolytic activity (60-70%) under the conditions of PHS at 0°C than at 37°C. Under the deglycerolization conditions of human erythrocytes, which were frozen to -196°С under the protection of glycerol (15%), chlorpromazine and trifluoperazine are able to reduce the hemolysis level of cells and show high antihemolytic activity (50-70%). A comparison of the results of the study of the effectiveness of chlorpromazine and trifluoperazine in a model experiment and in original conditions of removing glycerol from cells allows proving the feasibility of using the PHS model. Treatment of erythrocytes with glucose changes the sensitivity of human erythrocytes to posthypertonic shock.
Product Description
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Yershov Sergij S.
Yershova Natalia A.
Nipot Olena Ye.
Orlova Natalia V.
Romazanov Viktor V.
Rudenko Sergij V.
Semenchenko Oleksandr Yu.
Chabanenko Olena O.
Shapkina Olga O.
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2024-01-08
