Registration Number
0224U001987,
(0121U108373)
, R & D reports
Title
Development of a modern system of monitoring, forecasting, specific prevention and control of virus-bacterial associations in poultry within the concept of "Single Health" in connection with globalization and climate change
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Розроблення сучасної системи моніторингу, прогнозування, специфічної профілактики та контролю вірус-бактеріальних асоціацій у сільськогосподарської птиці в рамках концепції «Єдиного здоров’я» у зв’язку зі світовою глобалізацією та змінами клімату
Head
Muzyka Denys V., Доктор ветеринарних наук
Rula Oleksandr M., Кандидат ветеринарних наук
Stehnii Borys T., Доктор ветеринарних наук
Registration Date
03-02-2024
Organization
National Scientific Center Institute of Experimental and Clinical Medicine
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The aim of the work is to determine the composition of virus-bacterial associations in chickens and turkeys under different technological conditions and to develop a modern system of monitoring, forecasting, prevention and control of these associations given current trends of globalization and climate change.
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During the reporting period, 1,557 samples of blood serum from farm poultry were examined (1,091 samples in ELISA, 466 samples in HIT), which were obtained during 2021-2023 from farms in seven regions of Ukraine. The level of immunity to IBV, IBD and ND corresponded to the standard (60-100%); the presence of antibodies to pathogens was established: ILT (from 0 to 100%), PMV (from 0 to 100%), M. gallisepticum (from 0 to 80.0%), M. synoviae (from 0 to 65.0%). Antibodies to avian influenza viruses (influenza A, subtypes H5 and H7) were not detected. Bacteriological studies of paternal material from 586 heads were also carried out. poultry of different ages and different types of productivity, including hatching waste, from farms in five regions of Ukraine. Five Salmonella isolates were isolated, and their antibiotic sensitivity was studied. A phylogenetic analysis of the gene region of selected ILT isolates (Sumy 6-11/19, A 04-12, B 59-11 and B 2-10) was carried out. The vaccine-like isolate B 2-10 (isolated from a vaccinated bird) was included in a large cluster in which both field and vaccine viruses are located. The ILT B 59-11 virus (isolated from unvaccinated chickens) is located in a separate cluster and is related to the field ILT virus that circulated in Brazil. During the development of the technological regulations for the production of an inactivated vaccine against hemophilosis of birds, optimal nutrient media were selected for the cultivation of production strains, an inactivant was selected, and three experimental samples of the vaccine were obtained using a mixture of antigens and different ratios of adjuvants. In laboratory conditions, the antigenic and immunogenic activity of the developed biopreparation in comparison with the commercial vaccine was established on experimental birds - the immunogenicity of the experimental samples was 80-100%. In order to work out the method of selection and transportation of samples for bacteriological research.
Product Description
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Yechkenko Ruslana V.
Veretsun Andrii L
Vlashchenko Anton S.
Kolesnik Olena S.
Miezinov Oleksandr S.
Martynenko Ganna A.
Muzyka Nataliya М.
Pavlenko Viktoriia M.
Popova Anastasiya V.
Reshetylo Olha Yu.
Stegniy Anton B.
Tkachenko Semen V.
Usova Larysa P.
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2024-02-03
