Registration Number
0225U000129,
(0120U100545)
, R & D reports
Title
Investigation of regularities of release of bioactive water-soluble substances from the plant and animal tissues under the influence of low temperatures
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Дослідження закономірностей вивільнення біологічно активних водорозчинних речовин з тканин рослинного та тваринного походження при дії низьких температур
Head
Schenyavsky Ivan Yo., к.б.н.
Registration Date
06-01-2025
Organization
Institute of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine
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Investigation of the effects of low temperatures on biologically active compounds during their release from natural raw materials in order to increase the efficiency and extend the scope of their action and introduction into practical medicine
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The object of the study is cryoextracts of human cord blood, cow placenta and leaves of stinging nettle (Urtica dioica L.). The aim of the work is to investigate the effect of different modes of low-temperature destruction and extraction on the content of
some peptide and steroid hormones in extracts from tissues of animal (placenta and cord blood) and plant (nettle leaves) origin, as well as depending on the salt composition and pH of the extraction medium in comparison with disintegration and extraction in the temperature range of 70–100 ºС and after osmotic lysis. The following biochemical methods were used in the work: centrifugation, ultrafiltration, spectrophotometric, chromatographic, electrochemical and immunoenzymatic analysis. It was found that the use of "slow freezing-slow warming", 150 mM KCl and solutions with pH 5.0 are more effective. It has been shown that the use of freezing and subsequent thawing for destruction and extraction provides a higher yield of water-soluble biologically active substances (hormones, vitamins, protein substances) from the placenta tissue of cows and human cord blood compared to the use of heating to 70 ºС and hypotonic lysis. It has also been established that cryoextracts of nettle leaves are not inferior or have higher antioxidant activity compared to extracts obtained using a boiling water bath.
Product Description
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Akhatova Yuliia S.
Chaplai Olena V.
Hulevskyi Oleksandr K.
Shcheniavskyi Ivan Yo.
Horina Olha L.
Moiseieva Nataliia M.
Zharkova Yevheniia Ye.
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2025-01-06
