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Registration Number

0217U000011, 0116U000186 , R & D reports

Title

To study the mechanisms of development of metabolic disorders in the experimental pulmonary emphysema and work out the possibility of their correction

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Head

Korzhov Vitaliy, Доктор медичних наук

Registration Date

05-01-2017

Organization

Institute of tuberculosis and pulmonology named by F. G. Yanovsky

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Object - the blood of white rats with experimental pulmonary emphysema (PE). Aim - to find out the nature of violations of the state of glutathione-dependent detoxification system and oxidant-antioxidant system of the blood and to justify the possibility of their drug correction in experimental PE. Methods - experimental, biochemical, histological, statistics. The mechanisms of metabolic disorders in rats with experimental pulmonary emphysema, which help to reveal the pathogenesis of this disease: the activation of lipid peroxidation, and, as a consequence, reducing the stability of erythrocyte membranes, inactivation of the redox system of glutathione, a violation in the antioxidant defense system: decrease in catalase activity and the amount of ceruloplasmin, reduced peroxide resistance of erythrocytes, which indicates a shift pro antioxidant balance, decrease of the sorption capacity of red blood cells,which indicates the energy deficit in the erythrocytes. For typical PE display endogenous metabolic intoxication, which consist of the advantages of the histone proteins and DNA degradation products in the "middle molecules" erythrocytes. The criteria of evaluation of the functional status of glutathione-dependent system blood detoxication in experimental PE, which are indicators of the activity of key enzymes of the glutathione system: glutathione reductase, glutathione transferase, glutathione peroxidase, a hemolysate of erythrocytes as a function of dose of papain in the dynamics of the disease, which allow assess the extent of violation of the system state of glutathione, decompensation of its functionality in experimental PE, reveal one of the mechanisms of development of biochemical abnormalities. Sphere of application - pulmonology

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Жадан Вікторія Миколаївна
Коржов Віталій Іванович
Олексинська Ольга Олександрівна
Осипенко Божена Олегівна
Русінчук Ярослав Іванович

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2020-04-02