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Registration Number

0221U103208, 0116U000268 , R & D reports

Title

To study the antigenic and genetic markers of different Brucella species for National brucellosis control system harmonizationof in compliance with the requirements of European and international standards

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Head

Zavhorodnii Andrii I, д.вет.н.
Obukhovska Olena V,

Registration Date

16-02-2021

Organization

National Scientific Center Institute of Experimental and Clinical Medicine

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The method of production and control of brucellosis S- and RS-antigens as well as R-antigen has been developed. Three experimental series of standard antigens were obtained - brucellosis S-antigen from strain B. abortus 19 and RS-antigen from B. abortus 7-26 with a concentration of 40 × 109 CFU / cm3 and 10 × 109 CFU / cm3, respectively, as well as brucellosis R-antigen from B. ovis 67 / B with a working titer of 1:50. A method of antigen production for hyperimmunization of animals and a scheme of laboratory animals’ hyperimmunization for obtaining sera have been developed. The technology of manufacturing and control of the reference standard for serological diagnosis of brucellosis has been developed. An experimental series of "Antigen for serological diagnosis of animal brucellosis in RBT" was prepared and its activity and specificity in RBT with Anti-Brucella abortus serum OIEISS, the National Serum Standard Anti-B. abortus (Poland), National Biological Standard NSC "IECVM" Anti-Brucella abortus serum were studied. Laboratory regulations for the production and control of antigen for  

Product Description

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Bolotin Vitaliy I.
Dragut Svitlana S.
Kalinichenko Tetiana V.
Kutsenko Valentyna A
Marchenko Nataliia V
Ramazanova Taisiia P
Solodjankin Oleksij S.

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2021-02-16