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Information × Registration Number 0222U004982, 0120U000299 , R & D reports Title Studying the features of the functioning of cancer stem cells in the culture of tumor spheroids (mammospheres) popup.stage_title Head Sydorenko Mykhailo V., Registration Date 01-12-2022 Organization State Institution Department of Biotechnical Problems of Diagnostics of the Institute of Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine popup.description2  As a result of the research, it was found that tumor cells in the culture of multicellular tumor spheroids (MSS) and multicellular tumor spheroids enriched with stem tumor cells (SMSC) characterize the phenotypic characteristics of the primary tumor to a greater extent than in the monolayer culture of the MCF-7 line according to due to the weakening of the expression of epithelial markers (EpCAM) and the cells acquiring features of stem tumor cells (CD133, nestin). An increased expression of tumor stem cell (TSC) markers characteristic of breast cancer (CD133, nestin) in BPS and zBPS was revealed, compared to the adherent culture of MCF-7 cells cultivated under standard conditions in a complete nutrient medium. It has also been shown that after 48 hours of incubation under standard conditions, the monolayer culture is sensitive to the action of antitumor drugs, namely, doxorubicin, methotrexate, cisplatin and salinomycin. A tendency to a decrease in the number of living cells in the population of monosharophous cell culture under the action of antitumor drugs was revealed in the following sequence: methotrexate → salinomycin → cisplatin → doxorubicin. It has been established that cells can acquire characteristics of stem cells depending on the conditions in which they are located and in which nutrient medium they are cultivated. When cells are incubated in low-adhesive conditions with the addition of various growth factors and in a medium depleted of serum, the number of cells with stem-like characteristics increases. Product Description popup.authors Perepelytsina Olena M Sydorenko Мychaylo V. Yastrebova Olena V. popup.nrat_date 2022-12-01 Close