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Інформація × Реєстраційний номер 2124U000562, Матеріали видань та локальних репозитаріїв Категорія Стаття Назва роботи Human mesenchymal stem cells increase <scp>LLC</scp> metastasis and stimulate or decelerate tumor development depending on injection method and cell amount Автор Дата публікації 01-01-2024 Постачальник інформації Інституційний репозитарій (електронний архів) Київського національного університету імені Тараса Шевченка Першоджерело https://ir.library.knu.ua/handle/15071834/5153 Видання John Wiley & Sons, Inc Опис This work has been partially supported by the National Key R&D Program of China (2021YFF0502900); National Natural Science Foundation of China (61835009/62127819); Shenzhen Key Laboratory of Photonics and Biophotonics (ZDSYS20210623092006020) and Shenzhen Science and Technology Program (JCYJ20220818100202005) and National Academy of Sciences of Ukraine (2.2.5.443). Mesenchymal stem cells (MSCs) being injected into the body can stimulate or decelerate carcinogenesis. Here, the direction of influence of human placenta‐derived MSCs (P‐MSCs) on the Lewis lung carcinoma (LLC) tumor development and metastatic potential is investigated in C57BL/6 mice depending on the injection method. After intramuscular co‐inoculation of LLC and P‐MSCs (LLC + P‐MSCs), the growth of primary tumor and angiogenesis are slowed down compared to the control LLC on the 15th day. This is explained by the fact of a decrease in the secretion of proangiogenic factors during in vitro co‐cultivation of an equal amount of LLC and P‐MSCs. When P‐MSCs are intravenously (i.v.) injected in the mice with developing LLC (LLC + P‐MSCs(i.v.)), the tumor growth and angiogenesis are stimulated on the 15th day. A highly activated secretion of proangiogenic factors by P‐MSCs in a similar in vitro model can explain this. In both the models compared to the control on the 23rd day, there is no significant difference in the tumor growth, while angiogenesis remains correspondingly decelerated or stimulated. However, in both the models, the total volume and number of lung metastases constantly increase compared to the control: it is mainly due to small‐size metastases for LLC + P‐MSCs(i.v.) and larger ones for LLC + P‐MSCs. The increase in the rate of LLC cell dissemination after the injection of P‐MSCs is explained by the disordered polyploidy and chromosomal instability, leading to an increase in migration and invasion of cancer cells. After LLC + P‐MSCs co‐inoculation, the tumor cell karyotype has the most complex and heterogeneous chromosomal structure. These findings indicate a bidirectional effect of P‐MSCs on the growth of LLC in the early periods after injection, depending on the injection method, and, correspondingly, the number of contacting cells. However, regardless of the injection method, P‐MSCs are shown to increase LLC aggressiveness related to cancer‐associated angiogenesis and metastasis activation in the long term. Додано в НРАТ 2024-12-09 Закрити