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Information × Registration Number 0204U000309, 0198U007708 , R & D reports Title Investigation of protein synthesis and the lipid messenger role in connection with cold and frost resistance physiology of cereals during plant ontogenesis popup.stage_title Head Major Pavlo Stepanovych, Registration Date 29-01-2004 Organization Institute of Plant Physiology and Genetics NASU popup.description2 Embryos and etiolated seedlings of different genotypes as well as etiolated seedlings and green plants of several winter wheat cultivars were the object of the study. The aim of the study was to investigate changes of lipids of membranes and particular peculiarities of protein synthesis at initial stages of cereal plant ontogenesis under conditions of low temperatures. Methods of investigation: radiolabeling (32P-orthophosphate, 35S-methionine, 14C-aminoacids), thin-layer chromatography of lipids and polyphosphatydylinositols, gas-liquid chromatography of fatty acids, liquid ion-exchange chromatography of inositol phosphates, polyacryl gel electrophoresis of proteins, radioautography, densitometry of gels and radiograms, mathematical methods of experimental design and statistical analysis. It was found that the increasing in concentration of the signal transduction second messenger, inositol-1,3,4-triphosphate, occurred in cells affected by cold treatment of plants. The intensity of the increasing inconcentration of core phospholipid groups and percentage of unsaturated fatty acids in organs of maize seedlings correlated with their cold resistance. Antioxidative activity of total lipid fraction inversely correlated with contents of malonic dialdehyde and diene conjugates in maize seedlings indicating the important role of endogenic antioxidants under cold influence. The increased synthesis of stress proteins affected by low or high temperature took place in seedlings after passing defined stages of their development. The revealed positive correlation between contents of certain boiling-stable proteins in winter wheat seedlings and frost resistance of cultivars indicates the important role of these proteins in adaptation of cereal plants to low temperatures. Product Description popup.authors popup.nrat_date 2020-04-02 Close