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Information × Registration Number 0204U003957, 0103U002337 , R & D reports Title Selective autophagic peroxisome degradation in yeasts popup.stage_title Head Cybirnyj Andrij Andrijovych, Registration Date 03-06-2004 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 Pichia pastoris is a unique model system for dissecting the molecular mechanisms controlling autophagic peroxisome degradation (pexophagy). We isolated a collection of mutants defective in pexophagy (pdg mutants) and developed an allyl alcohol-based selection scheme for the cloning of PDG genes by functional complementation. The aims of our research work were to study of PDG proteins by intracellular localisation detection and to look for other proteins, interacting with them (2) detection of pupatetive vacuole transport componetns desrupted in each mutants. Among cloned genes, PDG3 (UGT51B) is predicted to encode a sterol glucosyltransferase, whose product is ergosterol glucoside (SG). We observed that a P. pastoris pdg3 UV-induced and constructed deletion mutant are defective in micro- and macropexophagy, as well as in the glucose-induced degradation of cytosolic enzymes. The micropexophagic process in the pdg3 mutant is blocked at the stage of homotypic vacuolar fusion. At the same time, this mutant exhibits normal vacuole morphology, and the process of nitrogen starvation-triggered general (bulk) autophagy was only partially affected. pdg3 mutants were more sensitive to elevated pH and high extracellular ethanol concentrations relative to the wild-type strain. Studies aimed at further elucidating the molecular function of Pdg3p in membrane-bound processes are in progress. Product Description popup.authors popup.nrat_date 2020-04-02 Close