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Information × Registration Number 0208U002565, 0107U008549 , R & D reports Title Investigation of the special features of karyotype and mitotic checkpoint activity changes during establishment of cell lines of mammalian stem cells in vitro. popup.stage_title Head Lukash Liubov, Доктор біологічних наук Registration Date 01-02-2008 Organization Institute of molecular biology & genetics popup.description2 It has been carried out the detailed chromosomal analysis of novel cell lines of mammalian stem cells at different stages of in vitro establishment. It has been shown that appearance of hyperploide cells, Robertsonian translocations, microchromosomes and chromosome fragments in mouse embryonic germ cell populations as well as appearance of chromosome breaks and fragments, formation of dicentric chromosomes in human stem cells obtained from different sources are characteristics of beginning of karyotypic evolution of cells in vitro. Such karyotypic evolution of cells are directed to adaptation of cells to conditions of cultivation that unfortunately are accompanied by loss of pluripotency of stem cells and gain of in vitro neoplastic cell transformation properties. It has been shown the attenuation of the mitotic checkpoint in cells of lines which have karyotypic destabilization. Product Description popup.authors popup.nrat_date 2020-04-02 Close