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Information × Registration Number 0208U004454, 0107U008781 , R & D reports Title To elaborate biotechnological foundations of in vitro artificial fertilization and embriogenesis in maize. popup.stage_title Head Satarova Tatjana Mikolaevna, Доктор біологічних наук Registration Date 13-11-2008 Organization State higher educational entity "Ukrainian State University of Chemical Technology" popup.description2 Object of the investigation the evalution of basic principls of in vitro artificial fertilization and embriogenesis in maize. The aim of the given work is the characterization of the early and late stages of maize embryogenesis in vivo and in vitro culture. It was first determined that maize embryogenesis in vivo corresponds to Graminad-type of embryogenesis. Under the explantation in early terms after pollination and culturing of immature caryopses in isolated culture the violation of the structure of early proembryos takes place that can be connected with the alteration of gradient of nutrient substance uptake. The explantation of isolated immature embryos of late terms after pollinations in vitro leads to the exit from the rest and germination.The addition of 0,1 mg/l abscisic acid to the nutrient medium does not slow down the process of germination, the growth of the caryopsis in vitro. Firstly the equation of regression were accounted which characterized the correlation between morphological indices of female inflorescent, caryopses, embryo sacs in the period of early embryogenesis and were used in practice for the determination of stages of embryogenesis. The branch of application -agro-industrial sector, breeding enterprises. Product Description popup.authors popup.nrat_date 2020-04-02 Close