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Information × Registration Number 0211U004054, 0109U004723 , R & D reports Title Controlling the content of formaldehyde in air by biosensors based on formaldehyde dehydrogenase and its removal bioreactor with immobilized alcohol oxidase. popup.stage_title Head Gonchar Mykhailo, Кандидат педагогічних наук Registration Date 20-01-2011 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 The object of investigation: formaldehyde metabolising enzymes of the methylotrophic thermotolerant yeast Hansenula polymorpha: NAD+- and glutathionе dependent formaldehyde dehydrogenase and FAD-containing octameric enzyme alcohol oxidase. The aim of the project: to construct enzymatic sensor based on thermostable formaldehyde dehydrogenase and to developa laboratory prototype of enzymatic cartridge of bioreactor based on alcohol oxidase for bioconversion of airborne formaldehyde. The methods of investigation: microbiological, biochemical, physico-chemical, enzymological, analytical. It was isolated alcohol oxidase and formaldehyde dehydrogenase from over-producing yeast strains of Hansenula polymorpha. To construct bioreactor, corresponding strains and purified enzymes preparations were trans ferred to Israel partner. The effective methods for immobilization of alcohol oxidase and formaldehyde dehydrogenase, using traditional and nanomaterials were developed. Enzymatic method for formaldehyde assay based on highly-purified formaldehyde dehydrogenase was developed. Еnzymatic biosensor based on thermostable formaldehyde dehydrogenase was constructed and a continuous fluidized bed bioreactor was designed to enable the bioconversion of airborne formaldehyde by alcohol oxidase or H. polymorpha cells immobilized in calcium alginate gel. Product Description popup.authors Гайда Г.З. Гончар М.В. Демків О.М. Закальська О.М. Смуток О.М. popup.nrat_date 2020-04-02 Close