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Information × Registration Number 0211U005961, 0111U006628 , R & D reports Title Immunoproteomic analysis of autoantigenic repertoire of unusually indolent tumors associated with prominent lymphocytic infiltratrion. popup.stage_title Head Filonenko Valeriy Viktorovich, Доктор біологічних наук Registration Date 20-12-2011 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 Object of research: antigenic repertoire of medullary breast carcinoma and papillary thyroid carcinoma. Purpose - to clone and to obtain purified recombinant proteins of potential autoantigens associated with medullary breast carcinoma (MBC) and thyroid cancer. To test in allogeneic screening autoantigens associated with MBC and other types of cancer with cancer patients and healthy donors sera using ELISA method. To perform statistical analysis of obtained data.- Methods - biochemical, molecular, genetic, genetic engineering, statistical. The cloning was carried out and 17 recombinant antigens associated with medullary breast carcinoma and thyroid carcinoma were obtained: RAD50, TOP2B, FAM50A, LGal3BP, HMGN2, PARD3, SAP30BP, ANKRD11, SPP1, PDCL, PABCP4, RBPJ, LRRFIP1, NY-BR-1, NY-BR-62, NY-CO-58, NaPi2b, which were tested in allogeneic screening with sera of patients with different cancer types (n=132), and also with healthy donors sera (n=35) using ELISA method. Ten antigens (RAD50, FAM50A, PARD3, SAP30BP, SPP1, RBPJ, TOP2B, NY-BR-1, NY-BR-62, NY-CO-58), which have statistically significant diagnostic potential were selected. We proposed new antigenic panel from 8 out of 10 antigens, which can diagnose breast cancer with a sufficiently high specificity and sensitivity (89% and 71% respectively). Product Description popup.authors Кіямова Р.Г. Костянець О.І. Савінська Л.О. Хоруженко А.І. popup.nrat_date 2020-04-02 Close