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Information × Registration Number 0211U006671, 0109U004663 , R & D reports Title Adhesion, proliferation and multilineage differentiation of mesenchymal stem cells upon the three-dimensional culturing in macroporous matrices based on polymeric cryogels that contain drafted "anchor" nano-particles. popup.stage_title Head Petrenko A.Yu., Registration Date 09-03-2011 Organization Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine popup.description2 Studies were carried out on stromal cells, derived from human adult bone marrow (BM). After in vitro expansion cells demonstrated immunophenotype CD29+, CD44+, CD73+, CD90+, CD105+ and capacity to multilineage differentiation, that corresponds to specific for mesenchymal stem cell (MSC) properties. During two-dimensional co-culture of BM MSC and macroporous spongy matrices based on polymeric agarose or alginate cryogels (MACS) there was no appearance of the decrease in proliferative potential of cells. MSC weakly adhere to agarose or alginate MACS, but adhesive properties of MACS could be improved by the modification of cryogels by the addition of type A gelatin. The chemical modification of MACS, prepared by the coupling of "anchor" nanoparticles of type A gelatin to the pore surfaces of macroporous cryogels was more effective than simple incorporation of gelatin. It was obtained that one of the most important factors for the optimal realization of MSC proliferative potential during three-dimensional culture within MACS is the cell seeding step. Cell biology and tissue engineering. Product Description popup.authors Волкова Наталія Олександрівна Грищук Віктор Петрович Мазур Світлана Петрівна Петренко Олександр Юрійович Петренко Юрій Олександрович Петрова Надія Іванівна popup.nrat_date 2020-04-02 Close