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Information × Registration Number 0211U006677, 0106U002164 , R & D reports Title Theoretical analysis and experimental study of specific mechanism of cryoinjury and cryoprotection of cells stipulated by the peculiarities of their functioning popup.stage_title Head Gordiyenko Evgenij A., Registration Date 09-03-2011 Organization Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine popup.description2 The research aim is to determine the peculiarities of cell cryodamage and cryoprotection due to their functional specialization, geometric, mechanic and transport parameters. Research object: physico-mathematical modeling of processes, being present at low-temperature preservation of cell suspensions, experimental determination of transport characteristics of cell membranes, geometric and mechanic parameters, cryoresistance under different cooling regimens of human erythrocytes, rat hepatocytes, kidneys of golden hamster, SPEV cells, testes cells, mice ovaries and embryos, Saccharomyces cerevisiae yeast cells. Methods of physico-mathematical modeling, thermodynamics of non-reversible processes, classical theory of phase transformations, hydrodynamics of binding solutions, light low-angle scattering, ion-selective electrometry, light microscopy, tenzodilatometry, viscosimetry, osmometry, thermography, program cooling as well as common methods of cell functional state estimation. Derived physico-mathematical model, describing extra- and intracellular solutions state in cell suspension during its crystallization, enabling to obtain quantitative estimation for optimal regimen of its cooling taking into account the cell transport characteristics. Analysis of crystallization peculiarities of cryoprotectants' aqueous solutions was carried out. It is shown that crystallization of cryoprotectants' aqueous solutions cannot be described with phasal diagrams of eutectic type. There were formulated principals of unbalanced diagrams which response phasal transitions during regimes really using in practical cryobiology. Novelty: the built theory using calculation it is possible to forecast cooling optimal regimes and composition of cryoprotective medium for cells with different functional specialization which accelerate the working-out of different origin cell suspensions low-temperature preservation and decrease their value. Application field: cryobiology, cryomedicine. Product Description popup.authors Гордієнко Євген Олександрович Гордієнко Ольга Іванівна Давидова Олена Володимирівна Кірєєв Віталій Олександрович Кирилюк Ганна Леонидівна Коваленко Ігор Федорович Коваленко Світлана Євгенівна Кулєшова Лариса Георгіївна Македонська Валентина Олександрівна Осецький Олександр Іванович Попівненко Людмила Іванівна Рєзніков Віктор Іванович Розанов Леонід Федорович Смольянінова Євгенія Іванівна Тодрін Олександр Феліксович Холодний Віталій Сергійович popup.nrat_date 2020-04-02 Close