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Information × Registration Number 0211U013055, 0110U006192 , R & D reports Title Development of new approaches to combinational cancer enzymotherapy based on amino acids deprivation popup.stage_title Head Stasyk Oleh Volodymyrovych, Кандидат біологічних наук Registration Date 15-12-2011 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 It was shown that in contrast to leucine, lysine or methionine starvation, the impact of deprivation for arginine varies between different human malignant cell lines. This suggests that individual cancer cell types differ in the level of sensitivity to arginine starvation and that the mechanisms that underline these differences need to be further investigated. Obtained data revealed that among four studied amino acids deprivation for semiessential amino acid arginine and essential amino acid methionine had stronger inhibitory impact on cell growth and viability in comparison with deprivation for such essential amino acids as leucine or lysine. It was shown that the lack of arginine metabolites, polyamines and agmatine, was not the key reason of cell growth inhibition in arginine-free medium and did not determined the differences in the level of cancer cells sensitivity to arginine withdrawal. We also revealed that supplementation of arginine-free culture medium with nitric oxide donor enhanced the cytotoxic properties of arginine withdrawal against human malignant suspension cells. Product Description popup.authors Барська Марина Леонідівна Бобак Ярослав Петрович Маєвська Оксана Михайлівна Шуваєва Галина Юріївна popup.nrat_date 2020-04-02 Close