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Information × Registration Number 0211U013359, 0110U006121 , R & D reports Title Generation and characterization of moloclonal antibodies to oncogenic, mutant forms of fibroblast growth factor receptor (FGFR3 ) for diagnostics and immunotherapy of tumors popup.stage_title Head Filonenko Valeriy Viktorovitch, Доктор біологічних наук Registration Date 26-12-2011 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 The main goal was to obtain mAbs specific for mutant forms of the fibroblast growth factor receptor FGFR3, comprising an amino acid replacement S249C and R248C. To do this, using ішеувшкусеув mutagenesis o DNA encoding the mutant forms FGFR3/S249C were btained. On the basis of HEK293 cells stable cell lines expressing wild-type FGFR3 and its mutant forms have been created. HEK293/FGFR3 cells (S249C) were used for immunization of animals with different schemes. Analysis of immune mice titer confirmed the effectiveness of immunization and the presence of serum antibodies specific for FGFR3. With the use of hybridoma technology received a few clones that produce antibodies, which require further detailed analysis to determine their specificity. Product Description popup.authors Кіямова Рамзія Галямівна Маланчук Оксана Миколаївна Овчаренко Галина Вікторівна popup.nrat_date 2020-04-02 Close