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Information × Registration Number 0212U002676, 0109U000691 , R & D reports Title Heme-heme oxygenase system in adaptation of metabolism under oxidative stress and during modulation of NO-radicals level popup.stage_title Head Kaliman P.A., Registration Date 24-01-2012 Organization Kharkov National University named after V.N. Karazin popup.description2 The aim of this study was to investigate the role of NO-radicals in the regulation of heme-heme oxygenase system under oxidative stress caused by accumulation of free heme in mammal blood under effect of hemolytic agents. Subject of this investigation was the mechanisms of defensive reactions formation under effect of hemolytic agents to mammal organism. Heme oxygenase, tryptophan-2,3-dioxygenase, 5- aminolevulinate synthase, superoxide dismutase and catalase activities, TBA-reactive products, reduced glutathione, nitrites, protein carbonyl groups and total heme content in different organs and the absorption in Soret region of Wistar rats blood serum were studied. The hemin chloride administration in dose 1,5 mg/ 100 g was found to cause accumulation of the heme-containing products and their subsequent transport to liver. Under elevation of the total free heme content in liver biphase response of 5- aminolevulinate synthase activity and increase of heme oxygenase activity were obserwed. Pretreatment by N?-nitro-L-arginine normalized the free heme content and dynamic of 5- ALA synthase activity but didn't affect the rate of heme catabolism. The hemin chloride administration didn't cause significant changes in antioxidant-prooxidant status of rat liver, kidneys and spleen. It was established the alterations of some antioxidant-prooxidant parameters in spleen and kidneys which obserwed at firth hours of hemin chloride action were caused by nitric oxide production in NO-synthases reactions. The glycerol administration caused accumulation of the total heme in blood serum, liver and kidney. Pretreatment by L-arginine didn?t prevente heme oxygenase induction in liver and kidney after glycerol injection, but decreased of heme degradation rate in vivo. As a result the accumulation of total heme in spleen, liver and kidney was sharply elevated and heme synthesis de novo was decreased. The L-arginine administration didn?t effect on alterations of some prooxidant parameters, but modulated antioxidant enzyme activity in liver. The L-arginine injection didn?t cause the changes in antioxidant-prooxidant status of rat kidneys under glycerol model rhabdomyolysis. It was established that in vitro NO donor sodium nitroprusside cause reversible decrease of heme oxygenase activity in spleen and irreversible inhibition of enzyme activity in liver. Product Description popup.authors Нікітченко І. Филимоненко В. popup.nrat_date 2020-04-02 Close