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Information × Registration Number 0212U005549, 0110U000404 , R & D reports Title Study of the mechanisms of cryodamage and cryoprotection of immobilized cells to increase their preservation during cryopreservation and lyophilization popup.stage_title Head Vysekantsev Igor Pavlovich, Registration Date 12-12-2012 Organization Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine popup.description2 Research objects were yeasts Saccharomyces boulardii, Saccharomyces cerevisiae, bacteria Bifidobacterium bifidum, Lactobacilus bulgaricus, inoculated cell culture, SPEV lines. Research aim was to study the effect of cryopreservation and lyophilization on the integrity of immobilized cells. Research methods: cell culturing; light, luminescent, confocal microscopy; spectrophotometry; immobilization on/in carriers; programmable freezing and lyophilization. For the experiments there was used programmable freezer of objects; inverted, biological, laser scanning microscopes, centrifuge, spectrophotometer. There were designed effective cryopreservation regimens of microbe cells, immobilized on/in carriers and the possibility of lyophilization of probiotics immobilized on enterosorbents was shown. There was established the possibility of substituting blood serum in media for cryopreservation of eukariotic cells. Efficiency of the introduction into practice, branch. The obtained results will be used when creating the preparations of immobilized probiotics of the 4th generation. These preparations are used in medicine, veterinary, agriculture. Product Description popup.authors Абрафікова Л.Г. Ананьїна А.Є. Артуянц А.Ю. Бабінець О.М. Висеканцев І.П. Гуріна Т.М. Калашнікова М.М. Кощій С.В. Кудокоцева О.В. Марценюк В.П. Онасенко О.С. Пішко О.В. Петренко Т.П. Пономарьова В.Л. Сергієнко А.М. Шатілова Л.Є. Щеглов А.В. popup.nrat_date 2020-04-02 Close