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Information × Registration Number 0212U008115, 0109U007090 , R & D reports Title The investigation of the state of epithelium in oral mucosa and biology of basal cells during formation of transformation phenotype. popup.stage_title Head Sydorenko Mychail V., Registration Date 06-11-2012 Organization Department of biotechnical problems of diagnostic Institute of problems criobiology and criomedicine NAS Ukraine popup.description2 Aim. Investigation of the state of epithelium and biology of basal cells and expression of markers of the epithelial stem /progenitor cells p63, CK5/14 ; biomarkers p53, Bcl-2, Ki-67 in epithelial layers of normal oral mucosa, of precancerous lesions , in tumor-distant mucosa and tumors in patiens with OSCC and epidemiological risk. Metods . The biopsy specimens of oral mucosa and tumors were classified according to the UICC criteria. Tissue sections were immunohistochemically stained using monoclonal antibodies: for p63 (clone А4А), for C5/C14 (clone LL002 ) for p53 (clone DO-7), for Кі-67 (clone МІВ-1) ,for Всl-2 (clone 124), (Denmark) and counted according to epithelial layers as labeling index (LI, %). Results. Basal cells of normal epithelium of oral mucosa strongly expressed the marker proteins of epithelial stem /progenitor cells p63, CK5/14 as the predominant. In oral mucosa paties with epidemiological risk , precancerous lesions, in tumor-distant mucosa revaled the progression of histopathological phenotype (hyperplasia, dysplasia, carcinoma in situ, invasive carcinoma) and expression of proteins p63, CK5/14 in not characteristic suprabasal layers of epithelium of mucosa. The suprabasal expression of proteins p63, CK5/14, p53 correlated with grade of epithelial dysplasia and risk of oral cancer. The labeling index (LI, %) of p63, CK5/14, p53 cells were hight in invasive carcinomas . This data demonstrate the participate of cells that expression of markers of epithelial stem /progenitor cells in development of transformation phenotype. and biology of OSCCs. Conclusion.The immunohistochemicall labeling index of p63, CK5/14, p53 cells in suprabasal epithelial layers of oral mucosa can be used for differential diagnostics of grade of epithelial dysplasia and carcinoma in situ. Product Description popup.authors Кірєєва Світлана Сергіївна Юрченко Наталія popup.nrat_date 2020-04-02 Close