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Registration Number

0213U000319, 0111U000101 , R & D reports

Title

Study bakteriotropic and immunotropic of exogenous and endogenous biologically active substances of various form of environmental biotechnology medicinal leech production

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Head

Frolov Alexander Kirilovich, Доктор медичних наук

Registration Date

31-01-2013

Organization

Zaporozhye National University

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Purpose - evaluation bakteriotropic and immunotropic secrets of medicinal leech immunogenesis methods. The goal achieved by the following objectives: the analysis of properties of different trophic ekoform medical leeches in natural waters and in artificial feeding, obtaining antigens from the bodies Annelida; selection pure culture of Aeromonas hydrophila; bakfiltrate sterile, sterile culture samples of water from the medical leech, research bakteriotrophic influence of biologically active substances of the medicinal leech contained in the culture water and bacterial filtrate, analysis of the impact of endogenous and exogenous biologically active substances of the medicinal leech on the performance of isolated blood leukocytes intact and during the course of hirudotherapy the study of reaction intensity blast transformation of human blood lymphocytes to mitogens and antigens Annelida, analysis of the dynamics of sensitivity C-reactive protein in hirudotherapy. The objects of study were: saprophytic and pathogenic bacterial flora, blood leukocytes, biologically active substances of the medicinal leech. Methods: complete blood count; biotechnology - cupping-glass method of growing medicinal leech, microbiological methods, immunological methods leykoformula blood, lymphocytes phenotyping monoclonal antibody reaction blastic transformation method and cytomorphometric immunogenesis pathogenetic methods in assessing immunity developed under by prof. Frolov A.K. Research conducted using the technical equipment of the Department of Immunology and Biochemistry, Biological Faculty ZNU. According to the results of scientific work was first found that endogenous biologically active substances of the medicinal leech in vitro experiments with stabilizing apoptotic effect, in a study in vivo - homeostatic effect people and animals in hirudotherapy. In the digestive tract of the medicinal leech is dominated by one type of bacteria - bacterium endosymbiont of A. hydrophila. Physiological characteristics of the medicinal leech contribute to long-term storage of blood cell populations survivors in the microenvironment of the digestive system. Sterile bakfiltrate A. hydrophila and sample the culture of cultural water from under the medicinal leech, exhibit pronounced bacteriostatic effect on saprophytic and pathogenic bacterial flora. Scientific research results are used in the preparation of master, diplom, undergraduate, coursework, lectures and laboratory work (courses: "General Cytology", "Basic Immunology", "Histology") and specialization courses "Immunology and Biochemistry," "Method of optimization and immunological control sessions hirudotherapy" implemented by the Department of Therapy, Physiotherapy, Balneology and profpatolohiyi Zaporozhye Medical Academy of Postgraduate Education,"Phenotyping lymphocytes methods of spontaneous and dependent MCAT to CD-avidity structures rosette method","Method of assessing the state of the immune system in women different periods of ontogenesise" - the Department of Therapy, Clinical Pharmacology and Endocrinology of Zaporozhye Medical Academy of Postgraduate Education.

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Копійка Віра Вікторівна
Литвиненко Раїса Олександрівна
Мірошниченко Аліна Едуардівна
Процько Юлія Сергіївна
Сергієнко Ганна Павлівна
Федотов Євген Рудольфович
Фролов Олександр Кирилович

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2020-04-02