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Information × Registration Number 0214U008224, 0110U006082 , R & D reports Title Development and study of molecular and genetic characteristics of herbicide-resistant crops popup.stage_title Head Morgun Bogdan Volodymyrovych, Registration Date 19-12-2014 Organization Institute of cell biology and genetic engineering NAS of Ukraine popup.description2 The influence of kinetin, dicamba and picloram was studied on the regeneration of shoots from bread wheat callus. The techniques of biolistic and Agrobacterium-mediated transformation of maize and wheat in vitro were worked out. The optimal conditions for: i) Agrobacterium-mediated transformation of bread wheat in planta; and ii) morphogenic calli of bread wheat in vitro were defined. The transgenic nature of 13 independent phosphinothricin-resistant lines of maize plants (T0) of 6 genotypes that were obtained after biolistic transformation of immature embryos was proved. 31 maize plants of T1 generation of two genotypes were received and were resistant to phosphinothricin. After their self-pollination a total of 6562 seeds of T2 were collected and planted in greenhouse. Physiological experiments on treatment of transgenic plants with herbicide Basta® were conducted and stable, resistant to the herbicide line were selected. 3 phosphinothricin-resistant regenerated plants of white cabbage were received. Product Description popup.authors Бавол Андрій Васильович Банникова Марія Олександрівна Голубенко А.В. Горбатюк І.Р. Нітовська Ірина Олександрівна popup.nrat_date 2020-04-02 Close