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Information × Registration Number 0215U001373, 0112U007573 , R & D reports Title The development of non-specifik cell-based biosensor dbnaliella vidis to determine the concentrations of xenobiotoxic factors popup.stage_title Head Bozhkov A., Registration Date 16-02-2015 Organization Kharkov National University named after V.N. Karazin popup.description2 The object of study - coopersensitive and cooperresistant culture microalgae Dunaliella viridis Teodor. var. viridis f. euchlora, strain IBASU-A N 29 and cytotoxic factors of blood serum of different nature. The aim is to study of changes in the intensity of respiration cell biosensor, the formation of aggregates and cell shape microalgae D. viridis to make various concentrations of copper, lead, cadmium, immunomodulators, and cytotoxic factors of blood serum of patients with different pathologies. Research methods - determination of nucleic acids, proteins, atomic absorption spectrometry, polarographic methods, light microscopy, imunoturbidimetriya, photometry, statistical methods for evaluating results. It has been shown that the relationship between the functional activity of the genome, cell motility and the formation of cell aggregates allows the use of a culture of D. viridis as a promising cell biosensor. Detected the dose dependence of the time features of loss cell motility D. viridis in the presence of various metal ions can not only determine the borders of the toxicity of various substances, but also to detect the presence of certain toxic compounds in the aquatic environment. An algorithm is proposed the screening of blood serum for the presence of cytotoxic factors combined with the use of test systems D. viridis using automated calculation of indicators of cytotoxicity, which allows to evaluate possible deviations from normal physiological conditions (reversible and irreversible imunophysiological states). Based on these results, it is possible to predict the severity of physiological damage, to assess the effectiveness of drug treatment and to determine the toxicity of pharmaceuticals in vitro (including immunotropic) with subsequent extrapolation to humans Product Description popup.authors А.Божков А.Голтвянский М.Ковальова О.Лавінська popup.nrat_date 2020-04-02 Close