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Information × Registration Number 0215U004986, 0115U005044 , R & D reports Title Obtaining of genetically engineered immunoconjugates and their application for immunoaffinity chromatography and identification of target antigens popup.stage_title Head Gorbatiuk Oksana Borisivna, Кандидат біологічних наук Registration Date 23-12-2015 Organization Institute of Molecular Biology and Genetics popup.description2 The effective method for purification of antibodies specific to cell surface marker CD34 was developed. The method is based on the immobilization of recombinant CD34 through a His-tag on metal chelate affinity chromatographic medium. This immobilization method provides obtaining a highly specific antibody and not requires chemical modification of the chromatographic matrix. Chromatography support with immobilized antigen can be recovered in case of loss antigen activity. In addition, method for ScFv(СD34)-mCherry fusion protein renaturation from bacterial inclusion bodies was optimized and the possibility of its application for immunodetection was shown.5481 Product Description popup.authors Іродов Д.М. Окунєв O. В. Шпильова С. П. popup.nrat_date 2020-04-02 Close