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Information × Registration Number 0215U008630, 0110U000692 , R & D reports Title Peculiarities of the functioning of mTOR-dependent signaling pathways: the multiplicity of mTOR isoforms and the regulation of metabolic processes in the cell popup.stage_title Head Filonenko Valeriy Viktorovitch, Registration Date 30-12-2015 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 The combined effect of fibroblast NIH 3T3 paracrine factors at increased mTOR signaling leads to remodeling the cytoskeleton of HeLa cells. This manifests in reducing the migration potential of tumor cells in a test of the wound surface. The colocalization mTOR kinase with cytokeratins in epithelial cells, and with vimentin in normal human fibroblast cells was shown by immunocytochemistry. The interaction was confirmed by imunopresipitation and by PLA. The analysis of ribosomal protein S6 (S6K1) kinase isoforms - p70d15 p85d15 in stable cell lines, indicates that they have no influence on cell proliferation, migration, and survival in normal condition or during starvation. At the same time, by FACS analysis revealed a statistically significant reduction in the size HEK293 cells, expressing deleting forms kinase. Furthermore the overall level of protein synthesis was reduced in expression p85d15. Overespression of p85d15 can affect the overall level of protein biosynthesis, without playing a leading role in adapting to various cell stress. Product Description popup.authors Гаріфулін Олег Мінірович Кіямова Рамзія Галямівна Маланчук Оксана Миколаївна Овчаренко Галина Вікторівна Пальчевський Сергій Станіславович Савінська Лілія Олексіївна Скороход Олександр Миколайович Тихонкова Ірина Олександрівна Хоруженко Антоніа Іванівна Чередник Ольга Володимирывна popup.nrat_date 2020-04-02 Close