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Information × Registration Number 0216U001725, 0111U001196 , R & D reports Title Structure-functional features and proliferative potential of endocrine tissues under culture, cryopreservation and transplantation popup.stage_title Head Bondarenko Tatyana P., Registration Date 29-03-2016 Organization Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine popup.description2 A research object - suspensions, primary cell cultures and tissue fragments of the thyroid gland, pancreas, gonads and adrenal glands at hypothermic storage, cryopreservation and transplantation to the animals with experimentally induced hormonal deficiency of corresponding glands. An aim of work is the study of structural and functional properties of suspensions, primary cell cultures and tissue fragments of the thyroid gland, pancreas, gonads and adrenal glands at hypothermic storage, cryopreservation and transplantation to the animals with experimentally induced hormonal deficiency of corresponding glands. Research methods are enzymatic disaggregation of tissue, culturing, hypothermic storage and cryopreservation of cells, endocrine gland hormonal insufficiency modeling, transplantation, measurement of cortisol, T4, testosterone, estradiol, progesterone by radioimmunoassay, insulin by ELISA, supravital staining, flow cytometry, immunocytochemistry with specific antibodies, light and fluorescence microscopy, histological analysis, morphometric analysis, statistical analysis of results. Results and their novelty - it is first shown that the cells of neonatal thyroid gland, pancreas, gonads and adrenal glands have a high hormonal and proliferative potential in culture, which can be saved due to the development of a regimen of hypothermic storage and cryopreservation, and then can be used for the correction of hormonal deficiency by transplantation to the recipients with experimental endocrine pathologies. Efficiency of introduction - 1 patent was got. Fields of application are cryobiology, cryomedicine, biotechnology, endocrinology, transplantation, veterinary medicine. Product Description popup.authors Алабедалькарім Наталія Михайлівна Білявська Світлана Борисівна Богуславський Костянтин Ігорович Божкова Юлія Олегівна Божок Галина Анатоліївна Бондаренко Тетяна Петрівна Бородіна Наталія Павлівна Дудецька Галина Вадимівна Кірошка Вікторія Володимирівна Кірошка Катерина Валеріївна Легач Євген Іванович Павлюк Олег Михайлович Пахомов Олександр Віталійович Плаксіна Катерина Михайлівна Сидоренко Ольга Сергіївна Тамаріна Ірина Вікторівна Трутаєва Ірина Анатоліївна Устиченко Вікторія Дмитрівна popup.nrat_date 2020-04-02 Close