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Information × Registration Number 0216U002454, 0113U000952 , R & D reports Title Study of pathogenetic features of multiple organ failure in experimental kranioskeletnoyi injury and explore means of systemic correction methods of cell therapy popup.stage_title Head Gudyma Arsen Arsenovych, Registration Date 15-03-2016 Organization Horbachevsky Ternopil State Medical Academy popup.description2 Final report on research, 297 p., 220 fig. 46 tab., 68 sources. The object of study - markers of inflammation and disease dynamics traumatic different pеriods trauma and fetal nerve umoah correction cells. Purpose: to clarify pathogenetic patterns of systematic violations in severe combined kranioskeletal trauma and effectiveness of cryopreserved fetal nerve cells. It is shown that systemic manifestation experimental trauma is breach of antioxidant-prooxidant balance in the internal organs that are removed from the place of direct damage. Under these conditions, in the tissue of the liver, lung and heart increases the intensity of lipid peroxidation activity with the depletion of antioxidant enzymes superoxide dismutase and catalase with a maximum of 3 days post-traumatic period. After 14 days the activity of these enzymes significantly increased, which leads to a maximum reduction of TBA-active products. Subsequently disease process subsides, but there is an imbalance defense mechanisms towards pro-oxidant dominance, especially after 21. Proved basic similarity pathogenetic patterns acute period, the period of early and late manifestations of traumatic disease in experimental animals and humans. It is proved that combined kranioskeletal injury initiates activation of free radical oxidation of lipids, increase in proinflammatory cytokines, impaired protein metabolism, humoral immunity in animals at the stage of acute response to injury and the early period of traumatic disease that is a major cause damage to tissues and the development of systemic organ failure. Revealed that in addition caused bleeding in rats with injury worsens kranioskeletal course of traumatic disease in all studied periods after application kranioskeletal injury. The positive impact imposed cryopreserved fetal nerve cells on animals with kranioskeletal injury and a similar injury complicated by bleeding. Found that animals under the influence of cell therapy reduced mortality, improved structure and ultrastructure of the liver, kidneys and lungs, renewed activity of enzymatic and non-enzymatic units antioxidant system, protein synthesis function of the liver, increased antioxidant reserves, improved immunologic reactivity, decreased cytolytic syndrome and signs of inflammation. Experimentally proved that the most pronounced effect of the introduction of cryopreserved fetal nerve cells observed from 14 days after the application of the injury. It is proved that in response to kranioskeletal injury in the acute phase and during the early manifestations of traumatic disease significantly disrupted bile formation, biliary, Power-excretory functions of the liver and synthesize bile especially at 3 and 7 days post-traumatic period. Complications kranioskeletal additional trauma blood loss deepens and accelerates the phenomenon of liver dysfunction arising from the first day of post-traumatic period and in all periods of observation are very different from those indicators injured animals without blood loss. In terms of injury kranioskeletal without additional input of fetal blood loss of nerve cells causes the most studied parameters improve functional status, which is statistically significantly different from the animals without correction in 7 days after applying injuries. In terms kranio-keletal injury with blood loss more use of fetal nerve cells accompanied by such a fundamentally positive effect, but the dynamics of the studied parameters positive effect has been observed from the third day. Comparison of the effectiveness of fetal cell therapy in animals with kranioskeletal trauma and trauma complicated by blood loss, the amplitude variation first studied parameters showed higher effectiveness of the proposed. PATHOGENESIS, MULTIPLE ORGAN FAILURE, KRANIO-SKELETION TRAUMA, BLOOD LOSS, FETAL NERVE CELLS,LIVER,LUNGS, KYDNEYS, CELL THERAPY. The results published in the scientific literature. Product Description popup.authors Борис Руслан Миколайович Гудима Арсен Арсенович Заєць Тетяна Анатоліївна Козак Дарія Володимирівна Лісничук Наталія Євгенівна Марущак Марія Іванівна popup.nrat_date 2020-04-02 Close