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Information × Registration Number 0216U002456, 0113U000142 , R & D reports Title Development of new bioanalytical methods of L-, D-lactate and L-arginine analysis for diagnostics of some diseases and control of their treatment popup.stage_title Head Gonchar Mykhailo Vasyl'ovych, Кандидат педагогічних наук Registration Date 16-03-2016 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 The object of research: bioanalytical important enzymes, biosensor analysis, fluorescent analysis, enzymatic analysis, L-lactate, D-lactate, L-arginine, biological samples. The aim of investigations: A development of new bioanalytical methods for quantitative analysis of some biomarkers important for clinical diagnostics, sports medicine (for example, L- and D-lactate) and control of enzyme therapy (as a model was proposed L-arginine - target for arginine hydrolys by recombinant enzymes - arginase I and arginine deiminase). Methods: microbiological, biochemical, physical, chemical, enzymatic, biosensoric. The results of the work: - two variants of enzyme-fluorescent approaches of the L-arginine analysis - mono enzymatic and bi-enzymatic was developed. The proposed methods are based on: 1) fluorimetric detection of urea - the product of arginase-depended reaction of L-arginine hydrolysis at the interaction with 2,3- butanedione monoxime; 2) fluorescence monitoring the arhinase- and urease-catalyzed hydrolysis product - ammonium ions - the reaction with ortho-phthalic aldehyde. The possibility of using the developed bioanalytical methods for the analysis of L-arginine in the real samples of pharmaceuticals and biological fluids was demonstrated; - a new biosensor method of analysis of L-arginine using recombinant arginine deiminase of bacteria Mycoplasma hominis was developed. The biosensor is based on mono enzyme conversion of L-arginine to citrulline and ammonia with amperometric detection of ammonium ions using chemosensing layer of Nafion-Polyaniline complex Product Description popup.authors Іваш М. Ф. Гайда А. В. Гайда Г. З. Демків О. М. Закальська О. М. Закальський А.Є. Карковська М. І. Куцяба В. І. Нечай Г. І. Прокопів Т. М. Свінтозельська М. М. Серкіз Р. Я. Смуток О. В. Стасюк Н. Є. popup.nrat_date 2020-04-02 Close